PUBLICATIONS
2025
103. Lopes-Nunes, J.; Lopes, M.; Rosado, B.; Maocha, I.G.; Rolo, J.; Gaspar, C.; Pires, B.; Rosado, T.; Gallardo, E.; Palmeira-de-Oliveira, A.; et al. Vaginal Formulation Development: A Strategy Based on Aptamer-Guided Liposome for Human Papillomavirus-Induced Lesions. European Journal of Pharmaceutics and Biopharmaceutics 2025, 114693, doi:10.1016/j.ejpb.2025.114693.
102. Lourenço, P.; Cruz, C. G-Quadruplex Conformational Switching for MiR-155-3p Detection Using a Ligand-Based Fluorescence Approach. Biomolecules 2025, 15, 410, doi:10.3390/biom15030410.
101. Miranda, A.; Baptista, B.; Figueira, M.; Sousa, F.; Maia, C.J.; Socorro, S.; Cruz, C. Sensitive Fluorescent Detection of SARS-CoV-2 RNA Using an Enzymatic-Based Method. Spectrochim Acta A Mol Biomol Spectrosc 2025, 125766, doi:10.1016/j.saa.2025.125766.
100. Alves, A.; Miranda, A.; Zanin, I.; Richter, S.N.; Mergny, J.-L.; Cruz, C. I-Motif Formation in the Promoter Region of the B-MYB Proto- Oncogene. Int J Biol Macromol 2025, 139582, doi:10.1016/j.ijbiomac.2025.139582.
2024
99. Figueiredo, J.; Carreira-Barral, I.; Lourenço, P.; Miranda, A.; Lopes Nunes, J.; Quesada, R.; Laranjo, M.; Mergny, J.-L.; Cruz, C. Synthesis of 1,10‐phenanthroline‐2,9‐bistriazoles: Evaluation as G‐quadruplex Binders and Anti‐tumor Activity. ChemMedChem 2024, doi:10.1002/cmdc.202400591.
98. Riscado, M.; Mariano, L.; Cruz, C.; Pichon, C.; Sousa, F. Biophysical Characterization of MicroRNA Mixtures Based on Molecular Beacons. Biochem Biophys Res Commun 2024, 736, 150913, doi:10.1016/j.bbrc.2024.150913.
97. Paulo, A.; Oliveira, M.C.; Cabral Campello, M.P.; Gano, L.; Raposinho, P.; Belchior, A.; Mendes, E.; Silva, C.D.; Lopes-Nunes, J.; Cruz, C. Evaluation of a Radioiodinated G‐quadruplex Binder in Cervical Cancer Models. ChemMedChem 2024, doi:10.1002/cmdc.202400438.
96. Lopes-Nunes, J.; Cabral Campello, M.P.; Paulo, A.; Nastruzzi, C.; Oliveira, P.A.; Cruz, C. AT11-Guided Liposomes for Oral Cancer Cells: From Characterization towards in Vitro Evaluation. J Drug Deliv Sci Technol 2024, 106214, doi:10.1016/j.jddst.2024.106214.
95. Lopes-Nunes, J.; Oliveira, P.A.; Cruz, C. Nanotherapy for Human Papillomavirus-Associated Cancers: Breakthroughs and Challenges. Trends Pharmacol Sci 2024, doi:10.1016/j.tips.2024.07.004.
94. Lopes-Nunes, J.; Oliveira, P.A.; Cruz, C. Enhanced Targeted Liposomal Delivery of Imiquimod via Aptamer Functionalization for Head and Neck Cancer Therapy. Colloids Surf B Biointerfaces 2024, 114121, doi:10.1016/j.colsurfb.2024.114121.
93. Moreira, D.; Alexandre, D.; Miranda, A.; Lourenço, P.; Baptista, P. V.; Tomaz, C.; Lu, Y.; Cruz, C. Detecting Mir-155-3p through a Molecular Beacon Bead-Based Assay. Molecules 2024, 29, 3182, doi:10.3390/molecules29133182.
92. Maocha, I.; Rosado, B.; Lopes-Nunes, J.; Lopes, M.; Rolo, J.; Pires, B.; Gallardo, E.; Palmeira-de-Oliveira, A.; Martinez-de-Oliveira, J.; de Oliveira, R.P.; et al. Imiquimod-Loaded Nanosystem for Treatment Human Papillomavirus-Induced Lesions. Pharmaceutics 2024, 16, 864, doi:10.3390/pharmaceutics16070864.
91. Miranda, A.; Cucchiarini, A.; Esnault, C.; Andrau, J.-C.; Oliveira, P.A.; Mergny, J.-L.; Cruz, C. G-Quadruplex Forming Motifs in the Promoter Region of the B-MYB Proto-Oncogene. Int J Biol Macromol 2024, 132244, doi:10.1016/j.ijbiomac.2024.132244.
90. Gamboa, J.; Lourenço, P.; Cruz, C.; Gallardo, E. Aptamers for the Delivery of Plant-Based Compounds: A Review. Pharmaceutics 2024, 16, 541, doi:10.3390/pharmaceutics16040541.
89. Alexandre, D.; Fernandes, A.R.; Baptista, P. V.; Cruz, C. Evaluation of MiR-155 Silencing Using a Molecular Beacon in Human Lung Adenocarcinoma Cell Line. Talanta 2024, 126052, doi:10.1016/j.talanta.2024.126052.
88. Lourenço, P.; Miranda, A.; Campello, M.P.C.; Paulo, A.; Louis-Mergny, J.; Cruz, C. Targeting Proto-Oncogene B-MYB G-Quadruplex with a Nucleic Acid–Based Fluorescent Probe. Int J Biol Macromol 2024, 131055, doi:10.1016/j.ijbiomac.2024.131055.
87. Palma, E.; İçhedef, C.; Fernandes, C.; Belchior, A.; Raposinho, P.; Gano, L.; Miranda, A.; Moreira, D.; Cruz, C.; Pires, A.S.; et al. Targeting of G–Quadruplex DNA with 99mTc(I)/Re(I) Tricarbonyl Complexes Carrying Pyridostatin Derivatives. Chemistry – A European Journal 2024, doi:10.1002/chem.202400285.
86. Figueiredo, J.; Mergny, J.L.; Cruz, C. G-Quadruplex Ligands in Cancer Therapy: Progress, Challenges, and Clinical Perspectives. Life Sci 2024, 340, 122481, doi:10.1016/J.LFS.2024.122481
85. Lopes-Nunes, J.; Simões, P.; Moreira, D.; Leandro, K.; Nobre, R.J.; Pereira de Almeida, L.; Campello, M.P.C.; Oliveira, M.C.; Paulo, A.; Coutinho, A.; et al. RNA-Based Liposomes for Oral Cancer: From Biophysical Characterization to Biological Evaluation. Int J Biol Macromol 2024, 259, 129157, doi:10.1016/j.ijbiomac.2023.129157.
2023
84. Figueiredo, J.; Djavaheri-Mergny, M.; Ferret, L.; Mergny, J.L.; Cruz, C. Harnessing G-Quadruplex Ligands for Lung Cancer Treatment: A Comprehensive Overview. Drug Discov Today 2023, 28, 103808, doi:10.1016/J.DRUDIS.2023.103808
83. Moreira, D., Lopes-Nunes, J., Santos, F. M., Campello, M. P. C., Oliveira, M. C., Paulo, A., Tomaz, C., & Cruz, C. (2023). Assessment of Aptamer as a Potential Drug Targeted Delivery for Retinal Angiogenesis Inhibition. Pharmaceuticals, 16(5), 751 https://doi.org/10.3390/PH16050751
82. Santos, T., Silva, M., Imbert, L., Campello, M. P. C., Paulo, A., Amrane, S., Salgado, G. F., Cruz, C., & Cabrita, E. J. (2023). Structural Perspective into the Interaction of an Oncogenesis-Relevant pre-miRNA G-Quadruplex Ligand Carrier with the Protein Nucleolin. Chemistry – A European Journal, 29(39), e202301181. https://doi.org/10.1002/CHEM.202301181
81. Santos, F.M.; Ciordia, S.; Mesquita, J.; Cruz, C.; Sousa, J.P.C. e; Passarinha, L.A.; Tomaz, C.T.; Paradela, A. Proteomics Profiling of Vitreous Humor Reveals Complement and Coagulation Components, Adhesion Factors, and Neurodegeneration Markers as Discriminatory Biomarkers of Vitreoretinal Eye Diseases. Front Immunol 2023, 14, 634, doi:10.3389/FIMMU.2023.1107295.
80. Cruz, C.; Miranda, A.; Santos, T. Aptamer-Based Microfluidics for Circulating Tumor Cells. In Aptamers Engineered Nanocarriers for Cancer Therapy; Elsevier, 2023; pp. 403–432. https://doi.org/10.1016/B978-0-323-85881-6.00004-X
2022
79. Miranda, A.; Lopez-Blanco, R.; Lopes-Nunes, J.; Melo, A.M.; Campello, M.P.C.; Paulo, A.; Oliveira, M.C.; Mergny, J.-L.; Oliveira, P.A.; Fernandez-Megia, E.; et al. Gallic Acid–Triethylene Glycol Aptadendrimers Synthesis, Biophysical Characterization and Cellular Evaluation. Pharmaceutics 2022, 14, 2456, https://doi.org/10.3390/pharmaceutics14112456
78. Moreira, D.; Leitão, D.; Lopes-Nunes, J.; Santos, T.; Figueiredo, J.; Miranda, A.; Alexandre, D.; Tomaz, C.; Mergny, J.-L.; Cruz, C. G- Quadruplex Aptamer-Ligand Characterization. Molecules 2022, https://doi.org/10.3390/molecules27206781
77. Figueiredo, J.; Carreira-Barral, I.; Quesada, R.; Mergny, J-L; Cruz, C. Synthesis and evaluation of 2,9-disubstituted-1,10- phenanthroline derivatives as G-quadruplex binders. Bioorg. Med. Chem. 2022, doi: https://doi.org/10.1016/j.bmc.2022.116971
76. Santos, T.; Salgado, G.F.; Cabrita, E.J.; Cruz, C. Nucleolin: a binding partner of G-quadruplex structures. Trends Cell Biol. 2022, doi: https://doi.org/10.1016/j.tcb.2022.03.003
75. Santos, T.; Lopes-Nunes, J.; Alexandre, D.; Miranda, A.; Figueiredo, J.; Silva, M.S.; Mergny, J.-L.; Cruz, C. Stabilization of a DNA aptamer by ligand binding. Biochimie 2022, doi: https://doi.org/10.1016/j.biochi.2022.05.002
74. Santos, T.; Miranda, A.; Imbert, L.; Monchaud, D.; Salgado, G.F.; Cabrita, E.J.; Cruz, C. Targeting a G-quadruplex from let-7e pre- miRNA with small molecules and nucleolin. J. Pharm. Biomed. Anal. 2022, 215, 114757, doi: https://doi.org/10.1016/j.jpba.2022.114757
73. Alexandre, D; Teixeira, B; Rico, A; Valente, S; Craveiro, A; Baptista, PV; Cruz, C. Molecular Beacon for Detection miRNA-21 as a Biomarker of Lung Cancer. International Journal of Molecular Sciences. 2022; 23(6):3330. https://doi.org/10.3390/ijms23063330
72. Figueiredo, Joana; Peitinho, David; Campello, Maria Paula Cabral; Oliveira, Maria Cristina; Paulo, António; Mergny, Jean-Louis; Cruz, Carla. "Screening of Scaffolds for the Design of G-Quadruplex Ligands". Applied Sciences 12 4 (2022): 2170. http://dx.doi.org/10.3390/app12042170
71. Maocha, Izamara Gomes; Carvalho, Josué; Lopes-Nunes, Jéssica; Rosado, Tiago; Gallardo, Eugénia; Tomás, Mariana; Palmeira-de- Oliveira, Ana; et al. Corresponding author: Cruz, Carla. "Drug formulations for localized treatment of Human Papillomavirus- induced lesions". Journal of Pharmaceutical Sciences (2022): http://dx.doi.org/10.1016/j.xphs.2022.02.004
70. I. V. Ramos, Catarina; A. S. Almodôvar, Vitor; Candeias, Nuno; Santos, Tiago; Cruz, Carla; Graça P. M. S. Neves, M.; Tomé, Augusto C.. Cruz, Carla. "Diketopyrrolo[3,4c]pyrrole derivative as a promising ligand for the stabilization of G-quadruplex DNA structures". Bioorganic Chemistry (2022): 105703. http://dx.doi.org/10.1016/j.bioorg.2022.105703
69. Silva, Francisco; D’Onofrio, Alice; Mendes, Carolina; Pinto, Catarina; Marques, Ana; Campello, Maria Paula Cabral; Oliveira, Maria Cristina; et al. "Radiolabeled Gold Nanoseeds Decorated with Substance P Peptides: Synthesis, Characterization and In Vitro Evaluation in Glioblastoma Cellular Models". International Journal of Molecular Sciences 23 2 (2022): 617 http://dx.doi.org/10.3390/ijms23020617
68. Santos, T.; Miranda, A.; Imbert, L.; Jardim, A.; Caneira, C.R.F.; Chu, V.; Conde, J.P.; Campello, M.P.C.; Paulo, A.; Salgado, G.; Cabrita, E.J.; Cruz, C. Pre-miRNA-149 G-quadruplex as a molecular agent to capture nucleolin. Eur. J. Pharm. Sci. 2022, 169, 106093, https://doi.org/10.1016/j.ejps.2021.106093
2021
67. Carvalho, J.; Lopes-Nunes, J.; Figueiredo, J.; Santos, T.; Miranda, A.; Riscado, M.; Sousa, F.; Duarte, A.P.; Socorro, S.; Tomaz, C.T.; Felgueiras, M.; Teixeira, R.; Faria, C.; Cruz, C. Molecular Beacon Assay Development for Severe Acute Respiratory Syndrome Coronavirus 2 Detection. Sensors 2021, 21, 7015. https://doi.org/10.3390/s21217015
66. Figueiredo, J.; Santos, T.; Miranda, A.; Alexandre, D.; Teixeira, B.; Simões, P.; Lopes-Nunes, J.; Cruz, C. Ligands as Stabilizers of G- Quadruplexes in Non-Coding RNAs. Molecules 2021, 26, 6164. https://doi.org/10.3390/molecules26206164
65. Lopes-Nunes, J.; Agonia, A.S.; Rosado, T.; Gallardo, E.; Palmeira-de-Oliveira, R.; Palmeira-de-Oliveira, A.; Martinez-de-Oliveira, J.; Fonseca-Moutinho, J.; Campello, M.P.C.; Paiva, A.; Paulo, A.; Vulgamott, A.; Ellignton, A.D.; Oliveira, P.A.; Cruz, C. Aptamer- Functionalized Gold Nanoparticles for Drug Delivery to Gynecological Carcinoma Cells. Cancers 2021, 13,4038 https://doi.org/10.3390/cancers13164038
64. Santos, T.; Salgado, G.F.; Cabrita, E.J.; Cruz, C. G-quadruplexes and Their Ligands: Biophysical Methods to Unravel G- Quadruplex/Ligand Interactions. Pharmaceuticals 2021, 14, 769. https://doi.org/10.3390/ph14080769
63. Cadoni, E., Magalhães, P. R., Emídio, R.M., Mendes E., Vítor J., Carvalho, J., Cruz, C., Victor, B.L., Paulo A. New (Iso)quinolinyl-pyridin 2,6-dicarboxamide G-Quadruplex Stabilizers. A Structure-Activity Relationship Study, Pharmaceuticals 14(7), 669, 2021 https://doi.org/10.3390/ph14070669
62. Lopes-Nunes, J., Oliveira, P. A., Cruz, C., G-Quadruplex-Based Drug Delivery Systems for Cancer Therapy, Pharmaceuticals 14(7), 671, 2021. https://doi.org/10.3390/ph14070671
61. Palma, E., Carvalho J., Cruz, C., Paulo A., Metal-Based G-Quadruplex Binders for Cancer Theranostics, Pharmaceuticals 14(7), 605, 2021. https://doi.org/10.3390/ph14070605
60. Carvalho, J., Lopes-Nunes J., Vialet, B., Rosado, T., Gallardo E., Vale, J., Eloy C., Ferreira S., Palmeira-de-Oliveira R., Campello, M.P.C., Paulo, A., Barthélémy, P., Mergny, J-L., Salgado, G.F., Queiroz, J.A., Ellington, A. D., Cruz, C., Nanoaggregate-forming lipid-conjugated AS1411 aptamer AS a promising tumor-targeted delivery system of anticancer agents in vitro, Nanomedicine: Nanotechnology, Biology and Medicine 36, 1221, 2021. https://doi.org/10.1016/j.nano.2021.102429
59. Miranda, A., Santos, T., Largy, E., Cruz, C., Locking up the AS1411 Aptamer with a Flanking Duplex: Towards an Improved Nucleolin-Targeting, Pharmaceuticals, 14(2), 121, 2021. https://doi.org/10.3390/ph14020121
58. Miranda, A., Santos, T., Carvalho, J., Alexandre, D., Jardim, A., Caneira, C. R. F., Vaz V., Pereira, B., Godinho, R., Brito, D., Chu, V., Conde J.P., Cruz, C., Aptamer-based microfluidic assay to detect nucleolin in prostate cancer. Talanta, 2021. https://doi.org/10.1016/j.talanta.2020.122037
57. Figueiredo, J., Miranda, A., Lopes-Nunes, J., Carvalho, J., Alexandre, D., Valente, S., Mergny, J.-L., Cruz, C., 2021. Targeting nucleolin by RNA G-quadruplex-forming motif. Biochemical Pharmacology. https://doi.org/10.1016/j.bcp.2021.114418
2020
56. Carvalho J., Lopes-Nunes, J., Campello M. P. C., Paulo A., Milici J., Meyers C., Mergny J-L., Salgado G. F., Cruz C. Human Papillomavirus G-Rich Regions as Potential Antiviral Drug Targets. Nucleic Acid Therapeutics. 2020. https://doi.org/10.1089/nat.2020.0869
55. Pereira P., Barreira M., Cruz C., Tomás J., Luís Â., Pedro A. Q., Queiroz J.A., Sousa F. Brain-Targeted Delivery of Pre-miR-29b Using Lactoferrin-Stearic Acid-Modified-Chitosan/Polyethyleneimine Polyplexes. Pharmaceuticals, 2020 https://doi.org/10.3390/ph13100314
54. Cruz C., Pedro A. Q., Carvalho J., Santos T., Talhada D., Paiva A., Queiroz J.A., Andrade M., Pinto M., Montenegro L., Delgado L., Pereira P. Nucleolin as a potential biomarker for canine malignant neoplasia. Research in Veterinary Science, 2020 https://doi.org/10.1016/j.rvsc.2020.10.004
53. Lopes-Nunes, J., Lifante J., Shen Y., Ximendes E. C., Jaque D., Iglesias-de la Cruz C., Cruz C. Biological studies of an ICG-tagged aptamer as drug delivery system for malignant melanoma. European Journal of Pharmaceutics and Biopharmaceutics, 154, 228- 235, 2020. https://doi.org/10.1016/j.ejpb.2020.07.018
52. Santos, T., Miranda A., Campello, M. P. C., Paulo, A., Salgado, G., Cabrita, E. J., Cruz, C. Recognition of Nucleolin through interaction with RNA G-Quadruplex. Biochemical Pharmacology, 2020. https://doi.org/10.1016/j.bcp.2020.114208
51. Carvalho, J., Mergny, J.-L., Salgado, G. F., Queiroz, J. A. and Cruz, C. G-quadruplex, Friend or Foe : The Role of the G-quartet in Anticancer Strategies. Trends in Molecular Medicine, 2020. https://doi.org/10.1016/j.molmed.2020.05.002
2019
50. Lopes-Nunes, J., Carvalho, J., Figueiredo, J., Ramos, C. I. V., Lourenço, L. M. O., Tomé, J. P. C., G.P.M.S. Neves, M., Mergny, J.-L., Queiroz, J. A., Salgado, G. F., and Cruz, C. Phthalocyanines for G-quadruplex aptamers binding. Bioorganic Chemistry 103920, 2020. https://doi.org/10.1016/J.BIOORG.2020.103920
49. Carvalho, J., and Cruz, C. Forster resonance energy transfer for studying nucleic acids denaturation: A chemical and biological sciences laboratory experiment. Biochemistry and Molecular Biology Education, 2019. https://doi.org/10.1002/bmb.21353
48. Eddahmi, M., Moura, N. M. M., Bouissane, L., Faustino, M. A. F., Cavaleiro, J. A. S., Paz, F. A. A., Mendes, R. F., Figueiredo, J., Carvalho, J., Cruz, C., Neves, M. G. P. M. S., and Rakib, E. M. Synthesis and Biological Evaluation of New Functionalized Nitroindazolylacetonitrile Derivatives. ChemistrySelect 4, 14335–14342, 2019. https://doi.org/10.1002/slct.201904344
47. Santos, T., Pereira, P., Queiroz, J.A., Cruz, C., Sousa, F. Plasmid production and purification: An integrated experiment‐based biochemistry and biotechnology laboratory course. Biochemistry and Molecular Biology Education, 2019. https://doi.org/10.1002/bmb.21290
46. Santos, T., Pereira, P., Campello, M.P.C., Paulo, A., Queiroz, J.A., Cabrita, E., Cruz, C. RNA G-quadruplex as supramolecular carrier for cancer-selective delivery. European Journal of Pharmaceutics and Biopharmaceutics, 142, 473-479, 2019. https://doi.org/10.1016/j.ejpb.2019.07.017
45. Figueiredo, J., Lopes-Nunes, J., Carvalho, J., Antunes, F., Ribeiro, M., Campello, M.P.C., Paulo, A., Paiva, A., Salgado, G.F., Queiroz, J.A., Mergny, J.-L., Cruz, C., 2019. AS1411 derivatives as carriers of G-quadruplex ligands for cervical cancer cells. Int. J. Pharm. https://doi.org/10.1016/j.ijpharm.2019.118511
44. Carvalho, J., Santos, T., Carrilho, R., Sousa, F., Salgado, G.F., Queiroz, J.A., Cruz, C., 2019. Ligand screening to pre-miRNA 149 G- quadruplex investigated by molecular dynamics. J. Biomol. Struct. Dyn. https://doi.org/10.1080/07391102.2019.1632743
43. Carvalho, J., Paiva, A., Campello, M.P.C., Paulo, A., Mergny, J.-L., Salgado, G.F., Queiroz, J.A., Cruz, C., 2019. Aptamer-based targeted delivery of a G-quadruplex ligand in cervical cancer cells. Sci. Reports. https://doi.org/10.1038/s41598-019-44388-9
42. Carvalho, J., Lopes-Nunes, J., Lopes, A.C., Campello, M.P.C., Paulo, A., Queiroz, J.A., Cruz, C., 2019. Aptamer-guided acridine derivatives for cervical cancer. Org. Biomol. Chem. https://doi.org/10.1039/C9OB00318E
41. Ramos, C.I.V., Almeida, S.P., Lourenço, L.M.O., Pereira, P.M.R., Fernandes, R., Faustino, M.A.F., Tomé, J.P.C., Carvalho, J., Cruz, C., Neves, M.G.P.M.S., 2019. Multicharged Phthalocyanines as Selective Ligands for G-Quadruplex DNA Structures. Molecules. https://doi.org/10.3390/molecules24040733
40. Carvalho, J., Alves, S., Castro, M.M.C.A., Geraldes, C.F.G.C., Queiroz, J.A., Fonseca, C.P., Cruz, C., 2019. Development of a bioreactor system for cytotoxic evaluation of pharmacological compounds in living cells using NMR spectroscopy. J Pharmacol Toxicol Methods. https://doi.org/10.1016/j.vascn.2018.11.004
2018
39. Santos, T., Brito, A., Boto, R., Sousa, P., Almeida, P., Cruz, C., Tomaz, C., 2018. Influenza DNA vaccine purification using pHEMA cryogel support. Sep. Purif. Technol. 206, 192-198. https://doi.org/10.1016/j.seppur.2018.06.002
38. Carvalho, J., Quintela, T., Gueddouda, N.M., Bourdoncle, A., Mergny, J.-L., Salgado, G.F., Queiroz, J.A., Cruz, C., 2018. Phenanthroline polyazamacrocycles as G-quadruplex DNA binders. Org. Biomol. Chem. https://doi.org/10.1039/C8OB00247A
2017
37. Carvalho, J., Pereira, E., Marquevielle, J., Campello, M.P.C., Mergny, J.-L., Paulo, A., Salgado, G.F., Queiroz, J.A., Cruz, C., 2017. Fluorescent light-up acridine orange derivatives bind and stabilize KRAS-22RT G-quadruplex. Biochimie. https://doi.org/10.1016/j.biochi.2017.11.004
36. Costa, D., Costa, C., Caldeira, M., Cortes, L., Queiroz, J.A., Cruz, C., 2017. Targeting of Cellular Organelles by Fluorescent Plasmid DNA Nanoparticles. Biomacromolecules 18, 2928–2936. https://doi.org/10.1021/acs.biomac.7b00877
35. Ferreira, J., Santos, T., Pereira, P., Corvo, M.C., Queiroz, J.A., Sousa, F., Cruz, C., 2017. Naphthalene amine support for G-quadruplex isolation. Analyst 142, 2982–2994. https://doi.org/10.1039/c7an00648a
34. Gaspar, V.M., Cruz, C., Queiroz, J.A., Pichon, C., Correia, I.J., Sousa, F., 2017. Highly selective capture of minicircle DNA biopharmaceuticals by a novel zinc-histidine peptide conjugate. Sep. Purif. Technol. 174, 417–424. https://doi.org/10.1016/j.seppur.2016.10.054
33. Santos, T., Proença, Z., Queiroz, J.A., Tomaz, C., Cruz, C., 2017. Plasmid purification by using a new naphthalene tripodal support. Sep. Purif. Technol. 188, 81–89. https://doi.org/10.1016/j.seppur.2017.06.072
32. Carvalho, J., Queiroz, J.A., Cruz, C., 2017. Circular Dichroism of G-Quadruplex: a Laboratory Experiment for the Study of Topology and Ligand Binding. J. Chem. Educ. 94, 1547–1551. https://doi.org/10.1021/acs.jchemed.7b00160
31. Carvalho, J., Nottelet, P., Mergny, J.-L.J.-L., Queiroz, J.A., Salgado, G.F., Cruz, C., 2017. Study of the interaction between indole- based compounds and biologically relevant G-quadruplexes. Biochimie 135, 186–195. https://doi.org/10.1016/j.biochi.2017.02.005
30. Medeiros-Silva, J., Guédin, A., Salgado, G.F., Mergny, J.-L.J.L., Queiroz, J.A., Cabrita, E.J., Cruz, C., 2017. Phenanthroline-bis-oxazole ligands for binding and stabilization of G-quadruplexes. Biochim. Biophys. Acta - Gen. Subj. 1861, 1281– 1292. https://doi.org/10.1016/j.bbagen.2016.11.024
2016
29. Pereira, P., Cruz, C., Queiroz, J.A., Figueiras, A., Sousa, F., 2016. Analysis of pre-MIR-29b binding conditions to amino acids by using a surface plasmon resonance biosensor. Anal. Methods 8, 205–213. https://doi.org/10.1039/c5ay02329g
28. Carvalho, J., Ferreira, J., Pereira, P., Coutinho, E., Guédin, A., Nottelet, P., Salgado, G.F., Mergny, J.-L., Queiroz, J.A., Sousa, F., Cabrita, E.J., Cruz, C., 2016. Stabilization of novel immunoglobulin switch regions G-quadruplexes by naphthalene and quinoline-based ligands. Tetrahedron 72, 1229–1237. https://doi.org/10.1016/j.tet.2016.01.022
27. Santos, T., Pereira, P., Sousa, F., Queiroz, J.A., Cruz, C., 2016. Purification of supercoiled G-quadruplex pDNA for in vitro transcription. Sep. Purif. Technol. 163, 59–71. https://doi.org/10.1016/j.seppur.2016.02.036
26. Santos, T., Carvalho, J., Corvo, M.C., Cabrita, E.J., Queiroz, J.A.A., Cruz, C., 2016. L-tryptophan and dipeptide derivatives for supercoiled plasmid DNA purification. Int. J. Biol. Macromol. 87, 385–396. https://doi.org/10.1016/j.ijbiomac.2016.02.079
2015
25. Ferreira, S., Carvalho, J., Valente, J.F.A., Corvo, M.C., Cabrita, E.J., Sousa, F., Queiroz, J.A., Cruz, C., 2015. Affinity analysis and application of dipeptides derived from l-tyrosine in plasmid purification. J. Chromatogr. B Anal. Technol. Biomed. Life Sci. 1006, 47–58. https://doi.org/10.1016/j.jchromb.2015.10.025
24. Mota, É., Sousa, F., Queiroz, J.A., Cruz, C., 2015. Quantitative analysis of the interaction between l-methionine derivative and oligonucleotides. J. Biochem. 157, 261–270. https://doi.org/10.1093/jb/mvu073
23. Santos, D., Medeiros-Silva, J., Cegonho, S., Alves, E., Ramilo-Gomes, F., Santos, A.O., Silvestre, S., Cruz, C., 2015. Cell proliferation effects of calix[4]arene derivatives. Tetrahedron 71, 7593–7599. https://doi.org/10.1016/j.tet.2015.07.077
22. Ferreira, S., Carvalho, J., Valente, J.F.A., Corvo, M.C., Cabrita, E.J., Sousa, F., Queiroz, J.A., Cruz, C., 2015. Affinity analysis and application of dipeptides derived from L-tyrosine in plasmid purification. J. Chromatogr. B 1006, 47–58. https://doi.org/10.1016/j.jchromb.2015.10.025
21. Amorim, L.F. a., Sousa, F., Queiroz, J. a., Cruz, C., Sousa, Â., 2015. Screening of L-histidine-based ligands to modify monolithic supports and selectively purify the supercoiled plasmid DNA isoform. J. Mol. Recognit. 28, 349–358 https://doi.org/10.1002/jmr.2449
20. Cruz, C., Sousa, Â., Mota, É., Sousa, F., Queiroz, J. a., 2014. Quantitative analysis of histamine- and agmatine–DNA interactions using surface plasmon resonance. Int. J. Biol. Macromol. 70, 131–137. https://doi.org/10.1016/j.ijbiomac.2014.06.044
19. Mota, E., Sousa, F., Sousa, Â., Queiroz, J. a, Cruz, C., 2014. Molecular recognition of oligonucleotides and plasmid DNA by l- methionine. J. Mol. Recognit. 27, 588–96. https://doi.org/10.1002/jmr.2382
18. Cruz, C., Boto, R.E.F., Drzazga, A.K., Almeida, P., Queiroz, J. a., 2014. NMR screening of new carbocyanine dyes as ligands for affinity chromatography. J. Mol. Recognit. 27, 197–204. https://doi.org/10.1002/jmr.2351
2013
17. Cruz, C., Cairrão, E., Lourenço, O., Almeida, P., Verde, I., Queiroz, J.A., 2013. Polyazamacrocycles as Potential Antitumor Agents for Human Prostate Cancer Cells. Chem. Biol. Drug Des. 81, 517–526. https://doi.org/10.1111/cbdd.12098
16. Gaspar, V.M., Cruz, C., Queiroz, J.A., Pichon, C., Correia, I.J., Sousa, F., 2013. Sensitive detection of peptide-minicircle DNA interactions by surface plasmon resonance. Anal. Chem. 85, 2304–2311. https://doi.org/10.1021/ac303288x
15. Cruz, C., Sousa, A., Sousa, F., Queiroz, J.A.J.A., Sousa, Â., Sousa, F., Queiroz, J.A.J.A., Sousa, A., Sousa, F., Queiroz, J.A.J.A., 2013 Affinity analysis between immobilized l-arginine and plasmid isoforms provided by surface plasmon resonance. Anal. Methods 5, 1682–1686. https://doi.org/10.1039/C3AY40213D
14. Cruz, C., Cairrão, E., Lourenço, O., Almeida, P., Verde, I., Queiroz, J.A., 2013. Polyazamacrocycles as potential antitumor agents for human prostate cancer cells. Chem. Biol. Drug Des. 81, 517–26. https://doi.org/10.1111/cbdd.12098
13. Cruz, C., Santos, S.D., Cabrita, E.J., Queiroz, J. a., 2013. Binding analysis between L-histidine immobilized and oligonucleotides by SPR and NMR. Int. J. Biol. Macromol. 56, 175–180. https://doi.org/10.1016/j.ijbiomac.2013.02.012
2012
12. Cruz, C., Sousa, Â., Sousa, F., Queiroz, J. a., 2012. Study of the specific interaction between l-methionine chromatography support and nucleotides. J. Chromatogr. B Anal. Technol. Biomed. Life Sci. 909, 1–5. https://doi.org/10.1016/j.jchromb.2012.09.037
2011
11. Cruz, C., Cairrao, E., Silvestre, S., Breitenfeld, L., Almeida, P., Queiroz, J.A., 2011. Targeting of mitochondria-endoplasmic reticulum by fluorescent macrocyclic compounds. PLoS One 6, e27078. https://doi.org/10.1371/journal.pone.0027078
10. Cruz, C., Cabrita, E.J., Queiroz, J.A., 2011. Screening nucleotide binding to amino acid-coated supports by surface plasmon resonance and nuclear magnetic resonance. Anal. Bioanal. Chem. 401, 983–993. https://doi.org/10.1007/s00216-011-5124-y
9. Cruz, C., Cabrita, E.J., Queiroz, J.A., 2011. Analysis of nucleotides binding to chromatography supports provided by nuclear magnetic resonance spectroscopy. J. Chromatogr. A1218, 3559–3564. https://doi.org/10.1016/j.chroma.2011.03.055
8. Cruz, C., Boto, R.E.F., Almeida, P., Queiroz, J. a., 2011. Study of specific interaction between nucleotides and dye support by nuclear magnetic resonance. J. Mol. Recognit. 24, 975–980. https://doi.org/10.1002/jmr.1144
2010
7. Sousa, F., Cruz, C., Queiroz, J.A., 2010. Amino acids-nucleotides biomolecular recognition: from biological occurrence to affinity chromatography. J Mol Recognit 23, 505–518. https://doi.org/10.1002/jmr.1053
2009
6. Cruz, C., Cahsto, V., Delgado, R., Félix, V., 2009. Design of protonated polyazamacrocycles based on phenanthroline motifs for selective uptake of aromatic carboxylate anions and herbicides. Chem. - A Eur. J. 15, 3277–3289. https://doi.org/10.1002/chem.200800993
2007
5. Cruz, C., Delgado, R., Drew, M.G.B., Félix, V., 2007. Evaluation of the binding ability of a novel dioxatetraazamacrocyclic receptor that contains two phenanthroline units: selective uptake of carboxylate anions. J. Org. Chem. 72, 4023–34 https://doi.org/10.1021/jo062653p
2005
4. David, S., Barros, V., Cruz, C., Delgado, R., 2005. In vitro effect of free and complexed indium(III) against Mycobacterium tuberculosis. FEMS Microbiol. Lett. 251, 119–124. https://doi.org/10.1016/j.femsle.2005.07.044
2004
3. Cruz, C., Delgado, R., Drew, M.G.B., Félix, V., 2004. Supramolecular aggregates between carboxylate anions and an octaaza macrocyclic receptor. Org. Biomol. Chem. 2, 2911–2918. https://doi.org/10.1039/b412059k
2003
2. Carvalho, S., Cruz, C., Delgado, R., Drew, M.G.B., Félix, V., 2003. Dinuclear copper and zinc complexes of a hexaazamacrocycle containing p-xylyl spacers and bridging anions: Theoretical and spectroscopic studies. Dalt. Trans. 4261–4270 https://doi.org10.1039/B306486G
1. Cruz, C., Carvalho, S., Delgado, R., Drew, M.G.B., Félix, V., Goodfellow, B.J., 2003. Metal complexes of a dipyridine octaazamacrocycle: Stability constants, structural and modelling studies. Dalt. Trans. 3172–3183 https://doi.org/10.1039/b304381a
